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Product Info Summary
| SKU: | M00196-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC-P, ICC |
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Product info
Product Name
Anti-CD80 Monoclonal Antibody [8G12]
SKU/Catalog Number
M00196-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CD80 Monoclonal Antibody [8G12] (Catalog # M00196-1). Tested in ELISA, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
CD80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CD80 Monoclonal Antibody [8G12] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00196-1)
Host
Mouse
Contents
CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 8G12
Isotype
IgG2b
Immunogen
CD80 antibody was raised against the extracellular domain of human CD80.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PD-L1 Antibody has no cross-reactivity to PD-L2.
Reactive Species
M00196-1 is reactive to CD80 in Human
Applications
M00196-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
33.048kDa
Background of B7-1/CD80
CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CD80 antibody can be used for immunohistochemistry starting at 2 - 5 μg/mL. For immunofluorescence start at 5 μg/mL. Flow cytometry at 10 μg/ml.
Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
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Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL.
Red: CD80 Antibody [8G12] (RF16041)
Blue: DAPI staining
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Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL.
Red: CD80 Antibody [8G12] (RF16041)
Blue: DAPI staining
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Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL.
Red: CD80 Antibody [8G12] (M00196-1)
Blue: DAPI staining
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Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.
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Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.
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Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.
Protein Target Info & Infographic
Gene/Protein Information For CD80 (Source: Uniprot.org, NCBI)
Gene Name
CD80
Full Name
T-lymphocyte activation antigen CD80
Weight
33.048kDa
Alternative Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80 CD80 B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 CD80 molecule T-lymphocyte activation antigen CD80|B-lymphocyte activation antigen B7|CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)|CTLA-4 counter-receptor B7.1|activation B7-1 antigen|costimulatory factor CD80|costimulatory molecule variant IgV-CD80
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD80, check out the CD80 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD80: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD80 Monoclonal Antibody [8G12] (M00196-1)
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No publications found for M00196-1
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